| 產(chǎn)品編號 | bs-60036R |
| 英文名稱 | Rabbit Anti-Acetyl-Histone H4 (Lys16) antibody |
| 中文名稱 | |
| 產(chǎn)品類型 | 乙酰化抗體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rice) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | Antigen affinity purification |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 組蛋白翻譯后修飾（PTMs）是表觀遺傳學調(diào)控染色質(zhì)結(jié)構(gòu)的關(guān)鍵機制，被稱為“組蛋白密碼”。組蛋白上的PTMs包括乙酰化、甲基化、磷酸化和新的酰化直接影響染色質(zhì)對轉(zhuǎn)錄因子的可及性和其他表觀遺傳調(diào)控，改變基因組的穩(wěn)定性，基因轉(zhuǎn)錄等。組蛋白乙酰化主要發(fā)生在核心組蛋白氨基末端的多個賴氨酸殘基上，以響應(yīng)各種刺激，在基因表達調(diào)控、DNA損傷修復、染色質(zhì)動力學等方面發(fā)揮重要作用。大多數(shù)情況下，組蛋白H2A主要在Lys5、9、15和36位處發(fā)生乙酰化修飾。 H2B主要在Lys5、12、15、16和20位處發(fā)生乙酰化修飾。組蛋白H3主要在Lys4、9、14、18、23、27、56和79位處發(fā)生乙酰化修飾。組蛋白H4主要在Lys5、8、12、16和20位處發(fā)生乙酰化修飾。組蛋白乙酰轉(zhuǎn)移酶（HATs）和組蛋白去乙酰化酶（HDACs）是主要的調(diào)節(jié)因子。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:400
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: Rice
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Human neuroblastoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:200
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bs-60036R
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:200
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bs-60036R
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
