| 產(chǎn)品編號 | Abk004 |
| 英文名稱 | Rabbit Anti-Tumor Immunity Characterization Antibody Assembly kit antibody |
| 中文名稱 | 腫瘤免疫表征分析抗體套裝 |
| 別 名 | Tumor immunity Characterization Antibody Assembly kit(CD4,CD8,PD-1,PD-L1,CTLA4) 腫瘤免疫表征分析抗體套裝(CD4,CD8,PD-1,PD-L1,CTLA4) |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) t-淋巴細胞 腫瘤細胞生物標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Recent clinical advances and basic discoveries have triggered a revolution in cancer immunotherapy. As a great breakthrough, some key immune checkpoints on the surface of tumor or immune cells were shown to play important roles in tumor immunity regulation and intervention of these targets will be beneficial to tumor therapy. Characterization of tumor infiltrating immune cells and tumor cells by examining immune cell phenotyping markers and immuno-checkpoints is important to efficacy of cancer immunotherapy. Among them, canonical helper T cell marker CD4, cytotoxic T cell marker CD8, PD-1 receptor on the surface of T cells, PD-1 ligand PD-L1 and CTLA-4 on the surface of tumor cells are top targets to be evaluated in tumor immunity research by different applications such as immuno-histochemistry (IHC) or Flow Cytometry (FCM). Tumor Immunity Characterization Antibody Assembly kit is one economical package of selected antibodies against CD4, CD8,PD-1, PD-L1 and CTLA4 respectively. These antibodies have been tested in a variety of applications and species. For specific application information of different antibodies, please refer to each individual antibody datasheet. Tumor Immunity Characterization Antibody Assembly kit Rabbit Anti-CD4 antibody (bs-0647R) Rabbit Anti-CD8 antibody (bs-0648R) Rabbit Anti-PD-1 antibody (bs-1867R) Rabbit Anti-PD-L1 antibody (bs-4941R) Rabbit Anti-CTLA4 antibody (bs-10006R) SWISS: N/A Gene ID: N/A Database links: Tumor Immunity Characterization Antibody Assembly kit Rabbit Anti-CD4 antibody (bs-0647R) Rabbit Anti-CD8 antibody (bs-0648R) Rabbit Anti-PD-1 antibody (bs-1867R) Rabbit Anti-PD-L1 antibody (bs-4941R) Rabbit Anti-CTLA4 antibody (bs-10006R)
基礎(chǔ)研究的不斷進步和最近的臨床進展引發(fā)了一場癌癥免疫治療的革命。作為一項重大突破，腫瘤或免疫細胞表面的一些關(guān)鍵免疫檢查點分子在腫瘤免疫調(diào)節(jié)中發(fā)揮著重要作用，對這些靶點進行干預(yù)將有利于腫瘤的治療。通過檢測免疫細胞表面標志和多種免疫檢查點的表達情況對腫瘤浸潤免疫細胞和腫瘤細胞表征進行分析，對腫瘤免疫治療的療效有重要意義。其中，經(jīng)典的輔助性T細胞標志CD4、細胞毒性T細胞標志CD8、T細胞表面表達的PD-1受體、腫瘤細胞表面表達的PD-L1（PD-1配體）和CTLA-4是在腫瘤免疫研究中利用免疫組織化學(xué)(IHC)或流式細胞術(shù)(FCM)進行分析的重要指標。腫瘤免疫表征分析抗體套裝極具性價比，包含了分別用于檢測CD4、CD8、PD-1、PD-L1和CTLA 4的抗體。這些抗體在多種應(yīng)用和物種中進行了測試。有關(guān)抗體具體使用信息，請參閱各個抗體產(chǎn)品數(shù)據(jù)表。 |
| 產(chǎn)品圖片 |
Primary: Anti- CD4(bs-0647R)
Sample:
HL60 Cell Lysate at 40 ug
Primary: Anti- CD4(bs-0647R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 55kD
Primary: Anti-PD-L1 (bs-4941R)
Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-PD-L1(bs-4941R)at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 32 kD
Primary: Anti-PD-1 (bs-1867R)
Sample:
Raji(Human) Cell Lysate at 30 ug
Thymus(Mouse) Lysate at 40 ug
Lymph node(Mouse) Lysate at 40 ug
Spleen(Mouse) Lysate at 40 ug
Raw264.7(Mouse) Cell Lysate at 40 ug
Thymus (Rat) Lysate at 40 ug
Primary: Anti-PD-1 (bs-1867R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 58 kD
Primary: Anti-CD8(bs-0648R)
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- CD8(bs-0648R)at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 36 kD
Primary: Anti-CTLA4(bs-10006R)
Sample: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-CTLA4 (bs-10006R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 21 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
