| 產品編號 | bs-9877R |
| 英文名稱 | Rabbit Anti-Staufen antibody |
| 中文名稱 | 雙鏈RNA結合蛋白Staufen抗體 |
| 別 名 | Double stranded RNA binding protein Staufen homolog 1; Double stranded RNA binding protein Staufen homolog; Double-stranded RNA-binding protein Staufen homolog 1; Double-stranded RNA-binding protein Staufen homolog; FLJ25010; MGC124588; STAU; STAU1; STAU1_HUMAN; staufen; Staufen RNA binding protein (Drosophila); Staufen RNA binding protein homolog 1. |
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Specific References (2) | bs-9877R has been referenced in 2 publications.
[IF=9.6] de Morrée, Antoine, et al. "Staufen1 inhibits MyoD translation to actively maintain muscle stem cell quiescence." Proceedings of the National Academy of Sciences 114.43 (2017): E8996-E9005. IF(ICC) ; Mouse.
[IF=9.504] Morree et al. Staufen1 inhibits MyoD translation to actively maintain muscle stem cell quiescence. (2017) Proc.Natl.Acad.Sci.U.S.A. 114:E8996-E9005 IF ; Human,?Mouse,?Rat,?Chicken,?Cow,?Horse,.
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| 研究領域 | 免疫學 神經生物學 細胞骨架 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Cow,Chicken,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Staufen/STAU1: 421-520/577 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
STAU1 (staufen, RNA binding protein, homolog 1) is a 577 amino acid protein that contains three double-stranded RNA-binding domains and is a mammalian homolog of Staufen, a Drosophila protein that is involved in mRNA transport during oogenesis and zygotic development. Localized to the rough endoplasmic reticulum (RER) and expressed in a variety of tissues, including heart, brain, liver, lung, pancreas, kidney and placenta, STAU1 binds to both Tubulin and double-stranded RNA and is thought to play an important role in mRNA transport from the microtubule network to the RER. Additionally, STAU1 may be involved in cross-linking cytoskeletal components with RNA, an event that is important for proper mRNA positioning during translation. Alternative splicing of the STAU1 gene yields two STAU1 isoforms, designated short and long. Function: Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site. Subunit: Binds tubulin. Binds with low affinity single-stranded RNA or DNA homopolymers. Interacts with CASC3 in an RNA-dependent manner (By similarity). Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with the influenza virus nonstructural protein NS1. Subcellular Location: Cytoplasm. Rough endoplasmic reticulum. Localizes exclusively with the rough reticulum endoplasmic. Tissue Specificity: Widely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta. Similarity: Contains 3 DRBM (double-stranded RNA-binding) domains. SWISS: O95793 Gene ID: 6780 Database links: Entrez Gene: 6780 Human Entrez Gene: 20853 Mouse Omim: 601716 Human SwissProt: O95793 Human SwissProt: Q9Z108 Mouse Unigene: 596704 Human Unigene: 73276 Mouse Unigene: 9028 Rat |
| 產品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- Staufen (bs-9877R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63/126 kD
Observed band size: 55 kD
Sample:
SW480(Human) Cell Lysate at 30 ug
Primary: Anti- Staufen (bs-9877R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63/126 kD
Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Staufen) Polyclonal Antibody, Unconjugated (bs-9877R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Staufen) Polyclonal Antibody, Unconjugated (bs-9877R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
