| 產品編號 | bs-4016R |
| 英文名稱 | Rabbit Anti-Histone H4 (Acetyl K8) antibody |
| 中文名稱 | 乙酰化組蛋白H4抗體 |
| 別 名 | Histone H4 (Acetyl K8); Histone H4 (Acetyl Lys8); Acetyl-Histone H4 (Lys8); Acetyl-Histone H4 (K8); H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN; Osteogenic growth peptide; OGP. |
| 產品類型 | 乙酰化抗體 |
| 研究領域 | 腫瘤 細胞生物 免疫學 發育生物學 染色質和核信號 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/test,ICC/IF=1:100,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 11kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised acetylpeptide derived from human Histone H4 around the acetylation site of Lys8: GG(Ac-K)GL |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008]. Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Subcellular Location: Nucleus. Chromosome. Post-translational modifications: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Sumoylated, which is associated with transcriptional repression. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Similarity: Belongs to the histone H4 family. SWISS: P62805 Gene ID: 8359 Database links: Entrez Gene: 121504 Human Entrez Gene: 554313 Human Entrez Gene: 8294 Human Entrez Gene: 8359 Human Entrez Gene: 8360 Human Entrez Gene: 8361 Human Entrez Gene: 8362 Human Entrez Gene: 8363 Human Entrez Gene: 8364 Human Entrez Gene: 8365 Human Entrez Gene: 8366 Human Entrez Gene: 8367 Human Entrez Gene: 8368 Human Entrez Gene: 8370 Human Entrez Gene: 100041230 Mouse Entrez Gene: 100862646 Mouse Entrez Gene: 319155 Mouse Entrez Gene: 319156 Mouse Entrez Gene: 319157 Mouse Entrez Gene: 319158 Mouse Entrez Gene: 319159 Mouse Entrez Gene: 319160 Mouse Entrez Gene: 319161 Mouse Entrez Gene: 320332 Mouse Entrez Gene: 326619 Mouse Entrez Gene: 326620 Mouse Entrez Gene: 69386 Mouse Entrez Gene: 97122 Mouse GenBank: NM_003548 Human Omim: 142750 Human SwissProt: P84040 Fruit fly (Drosophila melanogaster) SwissProt: P02304 Human SwissProt: P62805 Human SwissProt: P02304 Mouse SwissProt: P62806 Mouse SwissProt: P09322 Schizosaccharomyces pombe Unigene: 21500 Fruit fly (Drosophila melanogaster) Unigene: 29514 Fruit fly (Drosophila melanogaster) Unigene: 29527 Fruit fly (Drosophila melanogaster) Unigene: 30219 Fruit fly (Drosophila melanogaster) Unigene: 30220 Fruit fly (Drosophila melanogaster) Unigene: 30221 Fruit fly (Drosophila melanogaster) Unigene: 30223 Fruit fly (Drosophila melanogaster) Unigene: 30868 Fruit fly (Drosophila melanogaster) Unigene: 30869 Fruit fly (Drosophila melanogaster) Unigene: 30871 Fruit fly (Drosophila melanogaster) Unigene: 30872 Fruit fly (Drosophila melanogaster) Unigene: 30873 Fruit fly (Drosophila melanogaster) Unigene: 30876 Fruit fly (Drosophila melanogaster) Unigene: 33873 Fruit fly (Drosophila melanogaster) Unigene: 5747 Fruit fly (Drosophila melanogaster) Unigene: 143080 Human Unigene: 247816 Human Unigene: 248172 Human Unigene: 248178 Human Unigene: 248179 Human Unigene: 278483 Human Unigene: 352191 Human Unigene: 46423 Human Unigene: 528055 Human Unigene: 533295 Human Unigene: 55468 Human Unigene: 591790 Human Unigene: 655235 Human Unigene: 662174 Human Unigene: 706635 Human Unigene: 742244 Human Unigene: 14775 Mouse Unigene: 158272 Mouse Unigene: 227295 Mouse Unigene: 228709 Mouse Unigene: 246720 Mouse Unigene: 255646 Mouse Unigene: 260530 Mouse Unigene: 261642 Mouse Unigene: 261662 Mouse Unigene: 261664 Mouse Unigene: 377875 Mouse Unigene: 442307 Mouse Unigene: 486099 Mouse Unigene: 489077 Mouse |
| 產品圖片 |
Sample:
Lane 1: Human Hela cell lysates
Lane 2: Human Jurkat cell lysates
Lane 3: Human HL60 cell lysates
Lane 4: Human MCF-7 cell lysates
Primary: Anti-Histone H4 (Acetyl K8) (bs-4016R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 kD
Observed band size: 11 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H4 (Acetyl K8)) Polyclonal Antibody, Unconjugated (bs-4016R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H4 (Acetyl K8)) Polyclonal Antibody, Unconjugated (bs-4016R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Histone H4 (Acetyl K8)) polyclonal Antibody, Unconjugated (bs-4016R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control（black line）:NIH/3T3.
Primary Antibody (green line): Rabbit Anti-Histone H4 (Acetyl K8) antibody (bs-4016R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
