| 產品編號 | bs-0535R |
| 英文名稱 | Rabbit Anti-TFF3 antibody |
| 中文名稱 | 三葉肽因子3抗體 |
| 別 名 | Trefoil Factor 3; Trefoil factor3; hP1.B; Intestinal trefoil factor; ITF; mITF; OTTMUSP00000021729; Polypeptide P1.B; TFF3; TFI; TREFOIL; Trefoil factor (intestinal). |
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Specific References (2) | bs-0535R has been referenced in 2 publications.
[IF=4.36] Jiacheng Li. et al. Enhanced therapeutic effect on colitis with powder formulations of Painong San associated with the promotion of intestinal adhesion and absorption. J Ethnopharmacol. 2022 May;289:115030 IHC ; Mouse.
[IF=1.966] Wadey et al. Tissue expression and correlation of a panel of urinary biomarkers following cisplatin-induced kidney injury. (2013) Toxicol.Pathol. 42:591-602 IHC ; Human,?Mouse,?Rat,?Cow.
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| 研究領域 | 信號轉導 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Cow) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 6.5kDa |
| 細胞定位 | 細胞漿 細胞外基質 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TFF3: 51-80/94 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008] Subunit: Monomer. Homodimer; disulfide-linked. Subcellular Location: Secreted, extracellular space, extracellular matrix, apical lamina. Tissue Specificity: Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Similarity: Contains 1 P-type (trefoil) domain. SWISS: Q07654 Gene ID: 7033 Database links: Entrez Gene: 7033 Human Omim: 600633 Human SwissProt: Q07654 Human Unigene: 82961 Human 三葉草肽Ⅲ是三葉肽中結構獨特的一類小分子肽,它對于胃黏膜保護及損傷后修復具有重要作用,它的作用機制復雜,通過與其受體作用機制及信號傳導機制的研究,以加深其對胃黏膜保護機制的認識。在促進黏膜愈合的治療上,三葉肽是一個新的思路。 局部應用三葉肽可能使黏膜凝膠產生更大的穩定性,使胃黏膜對各種損傷因子的防御能力增強。三葉肽在誘導上皮修復上的直接作用,可用于許多胃腸道疾病。它作為輔助劑或取代皮質類固醇或其他免疫抑制劑，在潰瘍病及炎癥性腸病的治療上可能是一個突破。三葉肽研究可能對胃十二指腸潰瘍和炎癥、壞死性小腸結腸炎、放療和化療導致的黏膜炎帶來新的治療方法。 三葉草肽Ⅲ與黏液糖蛋白相互作用,可誘導上皮細胞遷移，參與信號傳導機制，調節胃酸及運動功能。 |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFF3 ) Polyclonal Antibody, Unconjugated (bs-0535R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (bs-0535R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (bs-0535R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (bs-0535R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (bs-0535R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TFF3 Polyclonal Antibody, Unconjugated(bs-0535R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
