| 產(chǎn)品編號 | bs-0795R |
| 英文名稱 | Rabbit Anti-Resistin antibody |
| 中文名稱 | 抵抗素抗體 |
| 別 名 | Adipose tissue specific secretory factor; ADSF; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1; Cysteine rich secreted protein A12 alpha like 2; Cysteine rich secreted protein FIZZ3; FIZZ 3; FIZZ3; Found in inflammatory zone 3; HXCP 1; HXCP1; MGC126603; MGC126609; PRO1199; Resistin delta2; RETN 1; RETN; RETN1; RSTN; UNQ407; XCP 1; XCP1 |
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Specific References (1) | bs-0795R has been referenced in 1 publications.
[IF=7.419] Hirona Kugo. et al. Tricaprin can prevent the development of AAA by attenuating aortic degeneration. BIOMED PHARMACOTHER. 2023 Apr;160:114299 IHC ; Rat.
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| 研究領域 | 心血管 細胞生物 免疫學 神經(jīng)生物學 信號轉(zhuǎn)導 生長因子和激素 激酶和磷酸酶 糖尿病 內(nèi)分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Mouse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Resistin: 21-114/114 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. [provided by RefSeq] Function: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Subcellular Location: Secreted. Tissue Specificity: Expressed only in fatty tissues. Similarity: Belongs to the resistin/FIZZ family. SWISS: Q9HD89 Gene ID: 56729 Database links: Entrez Gene: 56729 Human Entrez Gene: 57264 Mouse Omim: 605565 Human SwissProt: Q9HD89 Human SwissProt: Q99P87 Mouse Unigene: 283091 Human Unigene: 1181 Mouse 抵抗素Resistin, 是一種肽激素，富含半胱氨酸的分泌蛋白， 由脂肪細胞分泌的多肽類激素,屬于RELM家族，也稱之為ADSF（脂肪組織特異性的分泌因子）,抵抗素可能是肥胖和胰島素抵抗之間的重要鏈接。近期研究結果顯示,抵抗素 (Resistin)還是一種重要的脂肪組織旁分泌調(diào)質(zhì), 參與內(nèi)血管內(nèi)皮細胞功能穩(wěn)態(tài)的調(diào)節(jié), 在二型糖尿病心血管病變發(fā)生發(fā)展過程中發(fā)揮重要病理生理學作用. |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Resistin) Polyclonal Antibody, Unconjugated (bs-0795R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Resistin Polyclonal Antibody, Unconjugated(bs-0795R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
