| 產(chǎn)品編號 | bs-1030R |
| 英文名稱 | Rabbit Anti-HMGA1 antibody |
| 中文名稱 | 高遷移率族蛋白A1抗體 |
| 別 名 | HMG-I; HMG-Y; High mobility group AT-hook 1; HMG-I/HMG-Y; HMG-R; HMGA 1a; HMGA 1b; HMGA1; HMGI; HMGI/Y; HMGIY; HMGY; MGC12816; MGC4242; MGC4854; HMGA1_HUMAN; High mobility group protein HMG-I/HMG-Y; HMG-I(Y); AltName: Full=High mobility group AT-hook protein 1; High mobility group protein A1; High mobility group protein R. |
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Specific References (3) | bs-1030R has been referenced in 3 publications.
[IF=6.691] Jiang, Yiming. et al. Induction of Sestrin2 by pterostilbene suppresses ethanol-triggered hepatocyte senescence by degrading CCN1 via p62-dependent selective autophagy. 2021 Aug 17 IF ; Mouse.
[IF=4.741] Wang Shidong. et al. LncRNA CASC19 promotes gastric cancer progression through preventing CREB1 protein ubiquitin/proteasome-dependent degradation. CARCINOGENESIS. 2023 Jan;: WB ; Human.
[IF=2.43] Uchikura, Yuka, et al. "Extranuclear Translocation of High-Mobility Group A1 Reduces the Invasion of Extravillous Trophoblasts Involved in the Pathogenesis of Preeclampsia: New Aspect of High-Mobility Group A1." Reproductive Sciences (2017): 1933719117697254. IHC ; Human, Mouse.
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| 研究領(lǐng)域 | 染色質(zhì)和核信號 微生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Cow,Chicken) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HMGA1: 51-107/107 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
HMGA1 (HMGI/Y; high mobility group AT-hook 1) is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. There are two splice isoforms of HMGA1 - the full length HMGA1a/HMGI and the shortened form HMGA1b/HMGY. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. HMGA1 is frequently acetylated and is found in the nucleus. Interacts with HIV-1 pre-integration complex. Function: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. Subunit: Interacts with HIPK2 (By similarity). Interacts with HIV-1 pre-integration complex. Subcellular Location: Nucleus. Chromosome. Post-translational modifications: Constitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity. HMG-Y is not methylated. Methylation at Arg-58 is mutually exclusive with methylation at Arg-60. DISEASE: Note=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Similarity: Belongs to the HMGA family. Contains 3 A.T hook DNA-binding domains. SWISS: P17096 Gene ID: 3159 Database links: Entrez Gene: 3159 Human Entrez Gene: 15361 Mouse Omim: 600701 Human SwissProt: P17096 Human SwissProt: P17095 Mouse Unigene: 518805 Human Unigene: 4438 Mouse Unigene: 228608 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HMGA1/HMG-I/HMG-Y Polyclonal Antibody, Unconjugated(bs-1030R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HMGA1/HMG-I/HMG-Y Polyclonal Antibody, Unconjugated(bs-1030R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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